mouse kidney cancer cell line renca (ATCC)
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Mouse Kidney Cancer Cell Line Renca, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+kidney+cancer+cell+line+renca/Renca/pm38549430-29-8-19
Average 96 stars, based on 542 article reviews
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1) Product Images from "Swine pseudorabies virus attenuated vaccine reprograms the kidney cancer tumor microenvironment and synergizes with PD-1 blockade."
Article Title: Swine pseudorabies virus attenuated vaccine reprograms the kidney cancer tumor microenvironment and synergizes with PD-1 blockade.
Journal: Journal of medical virology
doi: 10.1002/jmv.29568
Figure Legend Snippet: FIGURE 1 The oncolytic activity of Pseudorabies virus live attenuated vaccine(PRV‐LAV) against kidney cancer in vitro. (A) Cell morphology of A498, Caki‐1, 786‐0, and Renca cells 48 h postinfection with PRV‐LAV. Phase‐contrast micrographs were captured using a Thermo EVOS M7000. Scale bars, 100 μm. (B) Cell viability assays were performed on A498, Caki‐1, 786‐0, and Renca cells 72 h after exposure to PRV‐LAV. Data are presented as the mean ± s.d. values (n = 5). A t‐test was used to determine the significance of differences between the PRV‐LAV infection group and the Mock group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (C) The expression of PRV gB in the infected cells was detected by immunofluorescence assay. Graphs were captured by Leica DMI8. Scale bars, 250 μm. (D) The expression of PRV gB was analyzed by western blot analysis after cancer cells were infected with PRV‐LAV (MOI = 1) and cultured for 0, 12, 24, 36, and 48 h. GAPDH was used as a loading control. Virus titer in the culture supernatant of A498 (E), Caki‐1 (F), 786‐0 (G), and Renca (H) cells post‐PRV‐LAV infection. Data are presented as the mean ± s.d. values (n = 4). PRV‐LAV, Pseudorabies virus live attenuated vaccine.
Techniques Used: Activity Assay, Virus, In Vitro, Infection, Expressing, Immunofluorescence, Western Blot, Cell Culture, Control
Figure Legend Snippet: FIGURE 2 The safety and therapeutic activity of Pseudorabies virus live attenuated vaccine (PRV‐LAV) against kidney cancer in vivo. (A) Timeline of the experimental setup for the experiments in the BALB/c mouse model. Weight curve (B) and survival rate curve (C) of mice after intravenous injection of vehicle or PRV‐LAV (4 × 107 PFUs) into the tail vein. Data are presented as the mean ± s.d. values (n = 6). (D) Timeline of the experimental setup for the experiments in the BALB/c nude mouse model. (E) Tumor volume curves of mice bearing A498 tumors treated with vehicle or PRV‐LAV (1 × 107 PFUs, intratumorally). Data are presented as the mean ± s.d. values (n = 6). (F) Timeline of the experimental setup for the experiments in the Renca syngeneic models. (G) Tumor volume curves of mice bearing Renca tumors treated with vehicle or PRV‐LAV (1 × 107 PFUs, intratumorally) with an initial tumor volume of approximately 100mm3. Data are presented as the mean ± s.d. values (n = 5). (H) Timeline of the experimental setup for the experiments in the Renca syngeneic models. (I) Tumor volume curves of mice bearing Renca tumors treated with vehicle or PRV‐LAV (1 × 107 PFUs, intratumorally) with an initial tumor volume of approximately 200 mm3. Data are presented as the mean ± s.d. values (n = 5). In (E, G, I), comparisons were performed by AUC analysis. Statistical analysis was performed by t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. PRV‐LAV, Pseudorabies virus live attenuated vaccine.
Techniques Used: Activity Assay, Virus, In Vivo, Injection